PubReading [78] - Megahertz serial crystallography - Max Wiedorn et al.

<p>The new <strong>European X-ray Free-Electron Laser</strong> is the first <strong>X-ray free-electron lase</strong>r capable of delivering X-ray pulses with a <strong>megahertz</strong> inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz <strong>serial femtosecond crystallography</strong> (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source. - DOI: 10.1038/s41467-018-06156-7 - 2018</p>