PubReading [299] - The Formation and Displacement of Ordered DNA Triplexes in Self- Assembled Three-Dimensional DNA Crystals - Y. Zhao, R. Sha et al.

<p>Reconfigurable structures engineered through DNA <strong>hybridization</strong> and self-assembly offer both structural and dynamic applications in nanotechnology. Here, we have demonstrated that strand displacement of triplex-forming oligonucleotides (TFOs) can be translated to a robust macroscopic DNA crystal by coloring the crystals with covalently attached <strong>fluorescent dyes</strong>. We show that three different types of triplex strand displacement are feasible within the DNA crystals and the bound TFOs can be removed and/or replaced by (a) changing the pH from 5 to 7, (b) the addition of the Watson–Crick complement to a <strong>TFO</strong> containing a short toehold, and (c) the addition of a longer TFO that uses the duplex edge as a toehold. We have also proved by X-ray diffraction that the structure of the crystals remains as designed in the presence of the TFOs.</p><p><a href="https://doi.org/10.1021/jacs.2c12667"><em>https://doi.org/10.1021/jacs.2c12667</em></a><em> - 2023</em></p>