PubReading [283] - PARP1 Gene Knockout Suppresses Expression of DNA Base Excision Repair Genes - A. Zakharenkoa, O. Lavrik et al.

<p>The effect of <strong><em>PARP1 </em></strong>knockout in HEK293 cells on the gene expression of DNA <strong>base excision repair</strong> (BER) proteins was studied. It was shown that the expression of all differentially expressed genes (DEGs) of BER was reduced by knockout. The expression of the DNA glycosylase gene <em>NEIL1</em>, which is considered to be one of the common “hubs” for binding BER proteins, has changed the most. The expression of genes of auxiliary subunits of DNA polymerases δ and ε is also significantly reduced. The <em>PARP1 </em>gene knock-out cell line obtained is an adequate cell model for studying the activity of the BER process in the absence of PARP1 and testing drugs aimed at <strong>inhibiting repair processes</strong>. It has been found for the first time that knock-out of the <em>PARP1 </em>gene results in a significant change in the level of expression of proteins responsible for <strong>ribosome biogenesis</strong> and the functioning of the proteasome.</p><p><em>DOI: 10.1134/S1607672922700028 - 2022</em></p>